Presentation Type
Poster Presentation
Abstract
This study assessed the absorption of key micronutrients from the multi-ingredient supplement using a novel developed LC-MS/MS method capable of analyzing B vitamins in a single run.
The study a randomized, double-blind, placebo-controlled crossover trial with 16 healthy participants (8 males, 8 females) to measure plasma nutrient appearance over 8 hours after supplement consumption. Serial blood samples were collected pre-consumption and at specified intervals post-ingestion. The novel LC-MS/MS method simultaneously quantified biotin, nicotinamide, pyridoxine, riboflavin, and thiamin in blood samples.
Results:The LC-MS/MS method had a range of 0.1 ng/mL to 1000 ng/mL and it proved to be robust and had good repeatability. An initial mobile phase of 100% water was necessary to provide separation for pyridoxine and thiamine. Good sensitivity was observed for the analytes. The nutritional supplement consumption resulted in significantly higher plasma AUC₀₋ₜ (p
Conclusions: Our findings demonstrate that micronutrients in the supplement are effectively absorbed into circulation following consumption. The developed LC-MS/MS method provided efficient, simultaneous analysis of B vitamins, confirming that complex powder blends of vitamins, minerals, probiotics, and phytonutrients are suitable formats for micronutrient supplementation without compromising nutrient bioavailability.
Faculty Mentor
Matthew J. Vergne
Recommended Citation
Brothers, Kennedy; Davis, Abigail; Losher, Anna; Ahmed, Yusra; Ghanem, Sora; and Vergne, Matthew J., "Have you had your vitamins today? Development of an LC-MS/MS method with a 100% aqueous gradient to simultaneously measure B vitamins in plasma from study participants in a “greens” nutritional supplement clinical trial" (2025). Student Scholar Symposium. 48.
https://digitalcollections.lipscomb.edu/student_scholars_symposium/2025/Full_schedule/48
Included in
Have you had your vitamins today? Development of an LC-MS/MS method with a 100% aqueous gradient to simultaneously measure B vitamins in plasma from study participants in a “greens” nutritional supplement clinical trial
This study assessed the absorption of key micronutrients from the multi-ingredient supplement using a novel developed LC-MS/MS method capable of analyzing B vitamins in a single run.
The study a randomized, double-blind, placebo-controlled crossover trial with 16 healthy participants (8 males, 8 females) to measure plasma nutrient appearance over 8 hours after supplement consumption. Serial blood samples were collected pre-consumption and at specified intervals post-ingestion. The novel LC-MS/MS method simultaneously quantified biotin, nicotinamide, pyridoxine, riboflavin, and thiamin in blood samples.
Results:The LC-MS/MS method had a range of 0.1 ng/mL to 1000 ng/mL and it proved to be robust and had good repeatability. An initial mobile phase of 100% water was necessary to provide separation for pyridoxine and thiamine. Good sensitivity was observed for the analytes. The nutritional supplement consumption resulted in significantly higher plasma AUC₀₋ₜ (p
Conclusions: Our findings demonstrate that micronutrients in the supplement are effectively absorbed into circulation following consumption. The developed LC-MS/MS method provided efficient, simultaneous analysis of B vitamins, confirming that complex powder blends of vitamins, minerals, probiotics, and phytonutrients are suitable formats for micronutrient supplementation without compromising nutrient bioavailability.