Presentation Type
Oral/Paper Presentation
Abstract
Objectives: This study assessed the absorption of key vitamins and other nutrients from amulti- ingredient “greens” nutritional supplement using a newly developed LC-MS/MS method capable of analyzing B vitamins in a single run.
Methods: We conducted a randomized, double-blind, placebo-controlled crossover trial with a number of healthy participants to measure plasma nutrient appearance over 8 hours after supplement consumption. Participants consumed either the nutritional supplement (13g) or placebo after a 10-hour fast, with treatments separated by a one-week. Serial blood samples were collected pre-consumption and at specified intervals post-ingestion. Our novel LC-MS/MS method simultaneously quantified biotin, nicotinamide, pyridoxine, riboflavin, and thiamin, while additional assays measured calcium, zinc, folate, and vitamin C. Area-under-the-curve (AUC₀₋ₜ) was calculated to assess nutrient bioavailability.
Results:The nutritional supplement consumption resulted in significantly higher plasma AUC₀₋ₜ (p
Conclusions: Our findings demonstrate that vitamins in the supplement are effectively absorbed into circulation following consumption. The developed LC-MS/MS method provided efficient, simultaneous analysis of B vitamins, confirming that complex powder blends of vitamins are suitable formats for micronutrient supplementation without compromising nutrient bioavailability.
Faculty Mentor
Matthew J. Vergne
Recommended Citation
Covington, Katelyn; Cravens, Joanna; Vergne, Matthew; and Akers, Scott, "Have you had your vitamins today? An LC-MS/MS method to simultaneously measure B vitamins in plasma from study participants in a “greens” nutritional supplement clinical trial." (2025). Student Scholar Symposium. 47.
https://digitalcollections.lipscomb.edu/student_scholars_symposium/2025/Full_schedule/47
Included in
Have you had your vitamins today? An LC-MS/MS method to simultaneously measure B vitamins in plasma from study participants in a “greens” nutritional supplement clinical trial.
Objectives: This study assessed the absorption of key vitamins and other nutrients from amulti- ingredient “greens” nutritional supplement using a newly developed LC-MS/MS method capable of analyzing B vitamins in a single run.
Methods: We conducted a randomized, double-blind, placebo-controlled crossover trial with a number of healthy participants to measure plasma nutrient appearance over 8 hours after supplement consumption. Participants consumed either the nutritional supplement (13g) or placebo after a 10-hour fast, with treatments separated by a one-week. Serial blood samples were collected pre-consumption and at specified intervals post-ingestion. Our novel LC-MS/MS method simultaneously quantified biotin, nicotinamide, pyridoxine, riboflavin, and thiamin, while additional assays measured calcium, zinc, folate, and vitamin C. Area-under-the-curve (AUC₀₋ₜ) was calculated to assess nutrient bioavailability.
Results:The nutritional supplement consumption resulted in significantly higher plasma AUC₀₋ₜ (p
Conclusions: Our findings demonstrate that vitamins in the supplement are effectively absorbed into circulation following consumption. The developed LC-MS/MS method provided efficient, simultaneous analysis of B vitamins, confirming that complex powder blends of vitamins are suitable formats for micronutrient supplementation without compromising nutrient bioavailability.